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KMID : 0603820110170020167
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Journal of Experimental & Biomedical Science
2011 Volume.17 No. 2 p.167 ~ p.171
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Increased Association of α-synuclein to Perturbed Cellular Membranes
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Kim Yoon-Suk
Lee Seung-Jae
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Abstract
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α-synuclein (α-syn) is implicated in the pathogenesis of Parkinson"s disease (PD) and other related diseases. We have previously reported that α-syn binds to the cell membranes in a transient and reversible manner. However, little is known about the physiologic function and/or consequence of this association. Here, we examined whether chemically induced perturbations to the cellular membranes enhance the binding of α-syn, based on hypothesis that α-syn may play a role in maintenance of membrane integrity or repair. We induced membrane perturbations or alterations in α-syn-overexpressing human neuroblastoma cells (SH-SY5Y) by treating the cells with hydrogen peroxide (H₂O₂) or oleic acid. In addition, membranes fractionated from these cells were perturbed by treating them with proteinase K or chloroform. Dynamic interaction of α-syn to the membranes was analyzed by the chemical cross-linking assay that we developed in the previous study. We found that membrane interaction of α-syn was increased upon treatment with membrane-perturbing reagents in a dose and time dependent manner. These results suggest that perturbations in the cellular membranes cause increased binding of α-syn, and this may have significant implication in the physiological function of α-syn in cells.
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KEYWORD
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α-synuclein, Membrane perturbations, Membrane interaction, Oxidative stress
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